RNA-seq10 topics

Differential Expression Guide

Step-by-step guidance for RNA-seq analysis in easyCris — from preparing count matrices to configuring RNA-seq models, interpreting results, and reading quality-control plots.

Stage 1

Data Preparation

Preparing a Count Matrix Designing Sample Metadata

Stage 2

Project Setup

Creating & Linking an RNA-seq Project

Stage 3

Model Configuration

Simple Model: ~condition Multi-factor Model: ~condition + batch Gene ID Lookup & Duplicate Handling

Stage 4

Advanced Models

Interaction Model: ~genotype * treatment Multi-run Comparator

Stage 5

Results & Interpretation

Interpreting the Results Table PCA & Quality Control Plots